E-ISSN 2602-4837
The role of Helicobacter pylori and gastroesophageal reflux disease in otitis media with effusion and evaluation of MUC4, MUC5B mucin genes and inflammatory cytokines in guinea pig middle ears [Tr-ENT]
Tr-ENT. 2020; 30(4): 131-139 | DOI: 10.5606/Tr-ENT.2020.22043

The role of Helicobacter pylori and gastroesophageal reflux disease in otitis media with effusion and evaluation of MUC4, MUC5B mucin genes and inflammatory cytokines in guinea pig middle ears

Emin Eren1, Mustafa Deniz Yılmaz2, Yeşim Çekin3, Arsenal Sezgin Alikanoğlu4, Hülya Eyigör5, Gamze Öztürk Yılmaz5, Ömer Tarık Selçuk5
1Department of Otorhinolaryngology, Private Academy Hospital, Konya, Turkey
2ENT Specialist, Mustafa Deniz Yılmaz Clinic, Antalya, Turkey
3Department of Microbiology, Antalya Training and Research Hospital, Antalya, Turkey
4Department of Pathology, Antalya Training and Research Hospital, Antalya, Turkey
5Department of Otorhinolaryngology, Antalya Training and Research Hospital, Antalya, Turkey

INTRODUCTION: This study aims to investigate the role of Helicobacter pylori (H. pylori) and gastroesophageal reflux disease (GERD) in otitis media with effusion (OME) and to examine the MUC4, MUC5B mucin genes in the guinea pig middle ears to analyze the inflammatory cytokines including tumor necrosis factor-alpha (TNF-a), interleukin (IL)-1beta (IL-1b), and IL-8.
METHODS: This study was conducted using eight guinea pigs. In the experimental group, H. pylori ATCC strain culture was injected transtympanically with microscopic guidance. The animals in the control group were injected with phosphate buffered saline (PBS) solution transtympanically. At 12, 24, 48, and 168 h after the injection, transtympanic PBS solution injection and aspiration of the middle ear were applied to all animals. After decapitation, the samples were taken.
RESULTS: In the histopathological examination, grading according to the degree of inflammation showed no statistically significant difference between the two groups (p>0.05). No statistically significant difference was determined in the analysis results of MUC4 and MUC5B genes in the middle ears of the two groups (p>0.05), the right and left ear values of IL-8, IL-1b, and TNF-a (p=0.999, p=0.610, and p=0.691, respectively).
DISCUSSION AND CONCLUSION: Based on our study results, there was no relationship between H. pylori injection in the middle ear and the histopathological changes of the middle ear mucosa. The results of the analysis of MUC4, MUC5B genes, and the inflammatory cytokines did not support the cause-and-effect relationship between H. pylori and OME.

Keywords: Guinea pig, interleukin-1beta, MUC4, MUC5B, otitis media with effusion, tumor necrosis factor-alpha

Emin Eren, Mustafa Deniz Yılmaz, Yeşim Çekin, Arsenal Sezgin Alikanoğlu, Hülya Eyigör, Gamze Öztürk Yılmaz, Ömer Tarık Selçuk. The role of Helicobacter pylori and gastroesophageal reflux disease in otitis media with effusion and evaluation of MUC4, MUC5B mucin genes and inflammatory cytokines in guinea pig middle ears. Tr-ENT. 2020; 30(4): 131-139

Corresponding Author: Gamze Öztürk Yılmaz, Türkiye
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