E-ISSN 2602-4837
The Turkish Journal of Ear Nose and Throat Detection of Epstein-Barr virus DNA by polymerase chain reaction in surgical specimens of patients with squamous cell carcinoma of the larynx and vocal cord nodules [Tr-ENT]
Tr-ENT. 2003; 11(5): 134-138

Detection of Epstein-Barr virus DNA by polymerase chain reaction in surgical specimens of patients with squamous cell carcinoma of the larynx and vocal cord nodules

Üzeyir Gök1, Aykut Özdarendeli, Erol Keleş, Yasemin Bulut, Bengü Çobanoğlu

Objectives: We investigated the incidence of Epstein-
Barr virus (EBV) infection in surgical specimens of squamous
cell carcinoma of the larynx and vocal cord nodules.
Patients and Methods: Formalin-fixed, paraffin- embedded
tissue samples randomly selected from 22 patients with squamous
cell carcinoma of the larynx (20 males, 2 females; mean
age 52 years) and from 17 patients with vocal cord nodules
(14 males, 3 females; mean age 38 years) were examined by
the polymerase chain reaction. The patients were assessed in
terms of localization of the disease, smoking habits, duration
of smoking, and the presence or absence of EBV DNA.
Results: Twenty-one patients with laryngeal carcinoma and
nine patients with vocal cord nodules were smokers.
Polymerase chain reaction showed EBV DNA in 11 patients
(50%) with laryngeal carcinoma and in seven patients
(41.2%) with vocal cord nodules. No significant differences
were found with respect to EBV DNA positivity, smoking
habits, smoking period, the number of cigarettes consumed
daily, localization of disease, and tumor stage (p>0.05).
Conclusion: Epstein-Barr virus does not seem to be
directly associated with the pathogenesis of laryngeal
squamous cell carcinoma, but its role in the etiology of
laryngeal proliferative diseases needs more elucidation.

Keywords: Carcinoma, squamous cell/virology; DNA, neoplasm;DNA, viral; Herpesvirus 4, human/isolation & purification; laryngealneoplasms/immunology; polymerase chain reaction/methods; vocalcords/ immunology.

Skuamöz hücreli larenks kanseri ve vokal kord nodüllü hastaların cerrahi doku örneklerinde polimeraz zincir reaksiyonuyla Epstein-Barr virüsü DNA’sı araştırması

Üzeyir Gök1, Aykut Özdarendeli, Erol Keleş, Yasemin Bulut, Bengü Çobanoğlu

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Anahtar Kelimeler: Karsinom, skuamöz hücreli/viroloji; DNA, neoplazi;DNA, viral; Herpesvirüs 4, insan/izolasyon ve purifikasyon;larenks neoplazileri/immünoloji; polimeraz zincir reaksiyonu/yöntem;vokal kord/immünoloji.

Üzeyir Gök, Aykut Özdarendeli, Erol Keleş, Yasemin Bulut, Bengü Çobanoğlu. Detection of Epstein-Barr virus DNA by polymerase chain reaction in surgical specimens of patients with squamous cell carcinoma of the larynx and vocal cord nodules. Tr-ENT. 2003; 11(5): 134-138

Corresponding Author: Üzeyir Gök
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